| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bacillus wiedmannii | ACCATGCGGTAAAGGGAGC | CCCCACCAAAATCCCATGG | 60.08 | 58.39 | 270 | 140 |
100.00%
|
100.00%
|
| Primer 2 | Bacillus wiedmannii | TCGAAGCACGTATGAAGGGTC | GCCATTCTGATCCTAATCGGC | 60.14 | 58.92 | 167 | 125 |
100.00%
|
100.00%
|
| Primer 3 | Bacillus wiedmannii | CGAAGCACGTATGAAGGGTC | GCCATTCTGATCCTAATCGGC | 58.72 | 58.92 | 166 | 125 |
100.00%
|
100.00%
|
| Primer 4 | Bacillus wiedmannii | ACAGCGGTTCGATTGGCA | CGGTTCCTGCACAACACA | 59.97 | 58.18 | 195 | 119 |
100.00%
|
100.00%
|
| Primer 5 | Bacillus wiedmannii | ATGCGGTAAAGGGAGCTGG | CCCCACCAAAATCCCATGG | 59.78 | 58.39 | 267 | 116 |
100.00%
|
100.00%
|
| Primer 6 | Bacillus wiedmannii | CGTAGCCGACGCAACTGTA | CCTTGCAAGCTTCCGTCGTA | 60.15 | 60.67 | 186 | 115 |
100.00%
|
100.00%
|
| Primer 7 | Bacillus wiedmannii | CGAAGCACGTATGAAGGGTCA | GCCATTCTGATCCTAATCGGC | 60.40 | 58.92 | 166 | 114 |
100.00%
|
100.00%
|
| Primer 8 | Bacillus wiedmannii | ACGTGAGCGGAAAACGGA | CGGTCGTTTGTCGTTTCCAC | 59.58 | 60.04 | 156 | 113 |
100.00%
|
100.00%
|
| Primer 9 | Bacillus wiedmannii | ACGTGAGCGGAAAACGGA | ACGGTCGTTTGTCGTTTCC | 59.58 | 58.70 | 157 | 113 |
100.00%
|
100.00%
|
| Primer 10 | Bacillus wiedmannii | ACGTGAGCGGAAAACGGA | CAAAACGTTCGTCTGCCGAT | 59.58 | 59.49 | 233 | 113 |
100.00%
|
100.00%
|